Nucleotide sequence of the gene coding for the major protein of hepatitis B surface antigen.

ABSTRACT: DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigen is a protein consisting of 226 amino acids and with a molecular weight of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences.

Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H.M. & Rutter W.J. Nucleotide sequence of the gene coding for the major protein of hepatitis B surface antigen. Nature 280, 215 (1979).

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